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A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis Editor100%: Meier, T.; Fahrenholz, F. and Fahrenholz, Falk: A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis Editor (ISBN: 9783034873512) 2012, in Englisch, Band: 62, Taschenbuch.
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9783034873512 - A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis (Paperback)
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A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis (Paperback) (2012)

Lieferung erfolgt aus/von: Vereinigtes Königreich Grossbritannien und Nordirland DE PB NW RP

ISBN: 9783034873512 bzw. 3034873514, in Deutsch, Springer Basel, Switzerland, Taschenbuch, neu, Nachdruck.

Fr. 92.31 ( 94.09)¹ + Versand: Fr. 1.33 ( 1.36)¹ = Fr. 93.65 ( 95.45)¹
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Von Händler/Antiquariat, The Book Depository EURO [60485773], London, United Kingdom.
Language: English Brand New Book ***** Print on Demand *****.2.1 Introduction .32 2.2 Technical Procedures .32 Chemical Synthesis .35 2.3 Results and Discussion.38 .Chemical Synthesis .38 Binding to anti-CCK antisera and CCK-B receptors .40 Binding to streptavidin agarose and photoelution .40 Affinity chromatography .41 2.4 Troubleshooting.43 References .44 .Purification of the Receptor for Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) using Biotinylated Ligands .45 Summary .45 3.1 Introduction .46 .3.2 Technical Procedures .48 Preparation of a Biotinylated Ligand .48 Preparation of Membrane Fraction .50 Solubilization of the PACAP Receptor .51 Partial Purification of the PACAP Receptor .52 Affinity Purification of the PACAP Receptor .54 Final Purification of the PACAP Receptor .56 Receptor-Binding Assay .57 3.3 Results and Discussion.58 .3.4 Troubleshooting .61 Impurity in PACAP27-Cys-NH.61 2 Low specific activity of the PACAP receptor despite a single band .62 Acknowledgments .62 References .63 .Photoreactive Biotinylated Peptide Ligands for Affinity Labeling . 65 Summary .65 4.1 Introduction.66 . 4.2 Technical Procedures . 67 Synthesis of a trifunctional photoactivatable biotinylating reagent .67 Synthesis of photo reactive biotinylated peptide hormones .67 Site-specific incorporation of biotin and photo labels in separate steps .69 Photoaffinity labeling .72 4.3 Results and Discussion .76 Synthesis of photoactivatable insulins with permanent biotin labels .76 Applications: Insulin .78 VI Examples for other applications .78 4.4 Troubleshooting.79 References .81 . Softcover reprint of the original 1st ed. 1996.
2
9783034873512 - A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis Biomethods
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A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis Biomethods

Lieferung erfolgt aus/von: Vereinigte Staaten von Amerika DE PB NW

ISBN: 9783034873512 bzw. 3034873514, in Deutsch, Birkhäuser, Taschenbuch, neu.

Fr. 84.67 ( 86.30)¹ + Versand: Fr. 3.48 ( 3.55)¹ = Fr. 88.15 ( 89.85)¹
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Von Händler/Antiquariat, BuySomeBooks [52360437], Las Vegas, NV, U.S.A.
Paperback. 260 pages. Dimensions: 9.6in. x 6.7in. x 0.6in.2. 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 2. 2 Technical Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 Chemical Synthesis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35 2. 3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 . . . . . . . . . . . . . Chemical Synthesis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 Binding to anti-CCK antisera and CCK-B receptors . . . . . . . . . . . . . . . . . . . . . . . . . . 40 Binding to streptavidin agarose and photoelution . . . . . . . . . . . . . . . . . . . . . . . . . . . 40 Affinity chromatography . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41 2. 4 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44 . . . . . . . . . . . . . . . . . Purification of the Receptor for Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) using Biotinylated Ligands . . . . . . . . . . . . . . . . . . . . . . . . . 45 Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45 3. 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46 . . . . . . . . . . . . . . . . . 3. 2 Technical Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 Preparation of a Biotinylated Ligand . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 Preparation of Membrane Fraction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50 Solubilization of the PACAP Receptor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51 Partial Purification of the PACAP Receptor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52 Affinity Purification of the PACAP Receptor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54 Final Purification of the PACAP Receptor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 Receptor-Binding Assay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57 3. 3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 . . . . . . . . . . . . . 3. 4 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61 Impurity in PACAP27-Cys-NH. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61 2 Low specific activity of the PACAP receptor despite a single band . . . . . . . . . . . . . 62 Acknowledgments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63 . . . . . . . . . . . . . . . . . Photoreactive Biotinylated Peptide Ligands for Affinity Labeling . . . . . . . . . 65 Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65 4. 1 Introduction. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66 . 4. 2 Technical Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . This item ships from multiple locations. Your book may arrive from Roseburg,OR, La Vergne,TN.
3
9783034873512 - Falk Fahrenholz: A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis
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Falk Fahrenholz

A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis (2012)

Lieferung erfolgt aus/von: Deutschland DE PB NW RP

ISBN: 9783034873512 bzw. 3034873514, in Deutsch, Birkhäuser Mrz 2012, Taschenbuch, neu, Nachdruck.

Fr. 83.93 ( 85.55)¹ + Versand: Fr. 15.21 ( 15.50)¹ = Fr. 99.14 ( 101.05)¹
unverbindlich
Von Händler/Antiquariat, AHA-BUCH GmbH [51283250], Einbeck, Germany.
This item is printed on demand - Print on Demand Titel. Neuware - 2.1 Introduction . 32 2.2 Technical Procedures . 32 Chemical Synthesis . 35 2.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 . . . . . . . . . . . . . Chemical Synthesis . 38 Binding to anti-CCK antisera and CCK-B receptors . 40 Binding to streptavidin agarose and photoelution . 40 Affinity chromatography . 41 2.4 Troubleshooting. 43 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44 . . . . . . . . . . . . . . . . . Purification of the Receptor for Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) using Biotinylated Ligands . 45 Summary . 45 3.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46 . . . . . . . . . . . . . . . . . 3.2 Technical Procedures . 48 Preparation of a Biotinylated Ligand . 48 Preparation of Membrane Fraction . 50 Solubilization of the PACAP Receptor . 51 Partial Purification of the PACAP Receptor . 52 Affinity Purification of the PACAP Receptor . 54 Final Purification of the PACAP Receptor . 56 Receptor-Binding Assay . 57 3.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 . . . . . . . . . . . . . 3.4 Troubleshooting . 61 Impurity in PACAP27-Cys-NH. 61 2 Low specific activity of the PACAP receptor despite a single band . 62 Acknowledgments . 62 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63 . . . . . . . . . . . . . . . . . Photoreactive Biotinylated Peptide Ligands for Affinity Labeling . ' 65 Summary . 65 4.1 Introduction. . . . . . . . . . 66 . 4.2 Technical Procedures . ' 67 Synthesis of a trifunctional photoactivatable biotinylating reagent . 67 Synthesis of photo reactive biotinylated peptide hormones . 67 Site-specific incorporation of biotin and photo labels in separate steps . 69 Photoaffinity labeling . 72 4.3 Results and Discussion . 76 Synthesis of photoactivatable insulins with permanent biotin labels . 76 Applications: Insulin . 78 VI Examples for other applications . 78 4.4 Troubleshooting. 79 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81 . . . . . . . . . . . . . . . . . . . 240 pp. Englisch.
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9783034873512 - A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis

A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis

Lieferung erfolgt aus/von: Kanada ~EN NW

ISBN: 9783034873512 bzw. 3034873514, Band: 62, vermutlich in Englisch, neu.

Fr. 89.12 (C$ 130.87)¹
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Lieferung aus: Kanada, Lagernd, zzgl. Versandkosten.
ceptor-Binding Assay ................................................. 57 3.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 58 . . . . . . . . . . . . . 3.4 Troubleshooting ....................................................... 61 Impurity in PACAP27-Cys-NH............................................ 61 2 Low specific activity of the PACAP receptor despite a single band ............. 62 Acknowledgments ..................................................... 62 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 63 . . . . . . . . . . . . . . . . . Photoreactive Biotinylated Peptide Ligands for Affinity Labeling ......... " 65 Summary ............................................................. 65 4.1 Introduction................................................ . . . . . . . .. . 66 . 4.2 Technical Procedures ................................................. " 67 Synthesis of a trifunctional photoactivatable biotinylating reagent ............. 67 Synthesis of photo reactive biotinylated peptide hormones ................... 67 Site-specific incorporation of biotin and photo labels in separate steps ........ 69 Photoaffinity labeling ................................................... 72 4.3 Results and Discussion .................................................. 76 Synthesis of photoactivatable insulins with permanent biotin labels ............ 76 Applications: Insulin .................................................... 78 VI Examples for other applications .......................................... 78 4.4 Troubleshooting....................................................... 79 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81 . . . . . . . . . . . . . . . . . . .
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9783034873512 - Herausgegeben von Meier, T. Fahrenholz, F.: A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis
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Herausgegeben von Meier, T. Fahrenholz, F.

A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis

Lieferung erfolgt aus/von: Deutschland DE PB NW

ISBN: 9783034873512 bzw. 3034873514, in Deutsch, Birkhäuser, Taschenbuch, neu.

Fr. 70.59 ( 71.95)¹
versandkostenfrei, unverbindlich
Lieferung aus: Deutschland, Versandkostenfrei.
buecher.de GmbH & Co. KG, [1].
2.1 Introduction ........................................................... 32 2.2 Technical Procedures ................................................... 32 Chemical Synthesis ..................................................... 35 2.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 38 . . . . . . . . . . . . . Chemical Synthesis ..................................................... 38 Binding to anti-CCK antisera and CCK-B receptors .......................... 40 Binding to streptavidin agarose and photoelution ........................... 40 Affinity chromatography ................................................. 41 2.4 Troubleshooting....................................................... 43 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 44 . . . . . . . . . . . . . . . . . Purification of the Receptor for Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) using Biotinylated Ligands ......................... 45 Summary ............................................................. 45 3.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . 46 . . . . . . . . . . . . . . . . . 3.2 Technical Procedures ................................................... 48 Preparation of a Biotinylated Ligand ...................................... 48 Preparation of Membrane Fraction ........................................ 50 Solubilization of the PACAP Receptor ..................................... 51 Partial Purification of the PACAP Receptor ................................. 52 Affinity Purification of the PACAP Receptor ................................ 54 Final Purification of the PACAP Receptor .................................. 56 Receptor-Binding Assay ................................................. 57 3.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 58 . . . . . . . . . . . . . 3.4 Troubleshooting ....................................................... 61 Impurity in PACAP27-Cys-NH............................................ 61 2 Low specific activity of the PACAP receptor despite a single band ............. 62 Acknowledgments ..................................................... 62 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 63 . . . . . . . . . . . . . . . . . Photoreactive Biotinylated Peptide Ligands for Affinity Labeling ......... " 65 Summary ............................................................. 65 4.1 Introduction................................................ . . . . . . . .. . 66 . 4.2 Technical Procedures ................................................. " 67 Synthesis of a trifunctional photoactivatable biotinylating reagent ............. 67 Synthesis of photo reactive biotinylated peptide hormones ................... 67 Site-specific incorporation of biotin and photo labels in separate steps ........ 69 Photoaffinity labeling ................................................... 72 4.3 Results and Discussion .................................................. 76 Synthesis of photoactivatable insulins with permanent biotin labels ............ 76 Applications: Insulin .................................................... 78 VI Examples for other applications .......................................... 78 4.4 Troubleshooting....................................................... 79 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81 . . . . . . . . . . . . . . . . . . .Softcover reprint of the original 1st ed. 1996. 2012. 244 mmVersandfertig in 3-5 Tagen, Softcover.
6
9783034873512 - Herausgegeben von Meier, T. Fahrenholz, F.: A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis
Herausgegeben von Meier, T. Fahrenholz, F.

A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis

Lieferung erfolgt aus/von: Deutschland DE PB NW

ISBN: 9783034873512 bzw. 3034873514, in Deutsch, Birkhäuser, Taschenbuch, neu.

Fr. 70.59 ( 71.95)¹
versandkostenfrei, unverbindlich
Lieferung aus: Deutschland, Versandkostenfrei.
buecher.de GmbH & Co. KG, [1].
ceptor-Binding Assay ................................................. 57 3.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 58 . . . . . . . . . . . . . 3.4 Troubleshooting ....................................................... 61 Impurity in PACAP27-Cys-NH............................................ 61 2 Low specific activity of the PACAP receptor despite a single band ............. 62 Acknowledgments ..................................................... 62 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 63 . . . . . . . . . . . . . . . . . Photoreactive Biotinylated Peptide Ligands for Affinity Labeling ......... " 65 Summary ............................................................. 65 4.1 Introduction................................................ . . . . . . . .. . 66 . 4.2 Technical Procedures ................................................. " 67 Synthesis of a trifunctional photoactivatable biotinylating reagent ............. 67 Synthesis of photo reactive biotinylated peptide hormones ................... 67 Site-specific incorporation of biotin and photo labels in separate steps ........ 69 Photoaffinity labeling ................................................... 72 4.3 Results and Discussion .................................................. 76 Synthesis of photoactivatable insulins with permanent biotin labels ............ 76 Applications: Insulin .................................................... 78 VI Examples for other applications .......................................... 78 4.4 Troubleshooting....................................................... 79 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81 . . . . . . . . . . . . . . . . . . .2012. xiv, 240 S. 244 mmVersandfertig in 3-5 Tagen, Softcover.
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9783034873512 - A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis T. Meier Editor

A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis T. Meier Editor

Lieferung erfolgt aus/von: Vereinigte Staaten von Amerika ~EN PB NW

ISBN: 9783034873512 bzw. 3034873514, Band: 62, vermutlich in Englisch, Birkhäuser Basel, Taschenbuch, neu.

Fr. 89.38 ($ 99.00)¹
unverbindlich
Lieferung aus: Vereinigte Staaten von Amerika, Lagernd, zzgl. Versandkosten.
2.1 Introduction ........................................................... 32 2.2 Technical Procedures ................................................... 32 Chemical Synthesis ..................................................... 35 2.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 38 . . . . . . . . . . . . . Chemical Synthesis ..................................................... 38 Binding to anti-CCK antisera and CCK-B receptors .......................... 40 Binding to streptavidin agarose and photoelution ........................... 40 Affinity chromatography ................................................. 41 2.4 Troubleshooting....................................................... 43 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 44 . . . . . . . . . . . . . . . . . Purification of the Receptor for Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) using Biotinylated Ligands ......................... 45 Summary ............................................................. 45 3.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . 46 . . . . . . . . . . . . . . . . . 3.2 Technical Procedures ................................................... 48 Preparation of a Biotinylated Ligand ...................................... 48 Preparation of Membrane Fraction ........................................ 50 Solubilization of the PACAP Receptor ..................................... 51 Partial Purification of the PACAP Receptor ................................. 52 Affinity Purification of the PACAP Receptor ................................ 54 Final Purification of the PACAP Receptor .................................. 56 Receptor-Binding Assay ................................................. 57 3.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 58 . . . . . . . . . . . . . 3.4 Troubleshooting ....................................................... 61 Impurity in PACAP27-Cys-NH............................................ 61 2 Low specific activity of the PACAP receptor despite a single band ............. 62 Acknowledgments ..................................................... 62 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 63 . . . . . . . . . . . . . . . . . Photoreactive Biotinylated Peptide Ligands for Affinity Labeling ......... 65 Summary ............................................................. 65 4.1 Introduction................................................ . . . . . . . .. . 66 . 4.2 Technical Procedures ................................................. 67 Synthesis of a trifunctional photoactivatable biotinylating reagent ............. 67 Synthesis of photo reactive biotinylated peptide hormones ................... 67 Site-specific incorporation of biotin and photo labels in separate steps ........ 69 Photoaffinity labeling ................................................... 72 4.3 Results and Discussion .................................................. 76 Synthesis of photoactivatable insulins with permanent biotin labels ............ 76 Applications: Insulin .................................................... 78 VI Examples for other applications .......................................... 78 4.4 Troubleshooting....................................................... 79 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81 . . . . . . . . . . . . . . . . . . .
8
9783034873512 - Herausgegeben von Meier, T. Fahrenholz, F.: A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis
Herausgegeben von Meier, T. Fahrenholz, F.

A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis

Lieferung erfolgt aus/von: Deutschland ~EN PB NW

ISBN: 9783034873512 bzw. 3034873514, Band: 62, vermutlich in Englisch, Springer, Basel Birkhäuser Basel, Taschenbuch, neu.

Fr. 63.76 ( 64.99)¹
versandkostenfrei, unverbindlich
Lieferung aus: Deutschland, Versandkosten nach: Deutschland, Versandkostenfrei.
Von Händler/Antiquariat, buecher.de GmbH & Co. KG, [1].
2.1 Introduction ........................................................... 32 2.2 Technical Procedures ................................................... 32 Chemical Synthesis ..................................................... 35 2.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 38 . . . . . . . . . . . . . Chemical Synthesis ..................................................... 38 Binding to anti-CCK antisera and CCK-B receptors .......................... 40 Binding to streptavidin agarose and photoelution ........................... 40 Affinity chromatography ................................................. 41 2.4 Troubleshooting....................................................... 43 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 44 . . . . . . . . . . . . . . . . . Purification of the Receptor for Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) using Biotinylated Ligands ......................... 45 Summary ............................................................. 45 3.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . 46 . . . . . . . . . . . . . . . . . 3.2 Technical Procedures ................................................... 48 Preparation of a Biotinylated Ligand ...................................... 48 Preparation of Membrane Fraction ........................................ 50 Solubilization of the PACAP Receptor ..................................... 51 Partial Purification of the PACAP Receptor ................................. 52 Affinity Purification of the PACAP Receptor ................................ 54 Final Purification of the PACAP Receptor .................................. 56 Receptor-Binding Assay ................................................. 57 3.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 58 . . . . . . . . . . . . . 3.4 Troubleshooting ....................................................... 61 Impurity in PACAP27-Cys-NH............................................ 61 2 Low specific activity of the PACAP receptor despite a single band ............. 62 Acknowledgments ..................................................... 62 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 63 . . . . . . . . . . . . . . . . . Photoreactive Biotinylated Peptide Ligands for Affinity Labeling ......... " 65 Summary ............................................................. 65 4.1 Introduction................................................ . . . . . . . .. . 66 . 4.2 Technical Procedures ................................................. " 67 Synthesis of a trifunctional photoactivatable biotinylating reagent ............. 67 Synthesis of photo reactive biotinylated peptide hormones ................... 67 Site-specific incorporation of biotin and photo labels in separate steps ........ 69 Photoaffinity labeling ................................................... 72 4.3 Results and Discussion .................................................. 76 Synthesis of photoactivatable insulins with permanent biotin labels ............ 76 Applications: Insulin .................................................... 78 VI Examples for other applications .......................................... 78 4.4 Troubleshooting....................................................... 79 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81 . . . . . . . . . . . . . . . . . . . 2012. xiv, 240 S. 244 mm Versandfertig in 6-10 Tagen, Softcover, Neuware, Offene Rechnung (Vorkasse vorbehalten).
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