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A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis (Biomethods)
alle Angebote vergleichen

Preise201320142015
SchnittFr. 86.40 ( 79.32)¹ Fr. 84.14 ( 77.24)¹ Fr. 97.10 ( 89.14)¹
Nachfrage
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A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis Biomethods (?)

ISBN: 9783034873512 (?) bzw. 3034873514, in Deutsch, Birkhäuser, Taschenbuch, neu

Fr. 94.01 ( 86.30)¹ + Versand: Fr. 3.87 ( 3.55)¹ = Fr. 97.87 ( 89.85)¹(unverbindlich)
Von Händler/Antiquariat, BuySomeBooks [52360437], Las Vegas, NV, U.S.A.
Paperback. 260 pages. Dimensions: 9.6in. x 6.7in. x 0.6in.2. 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 2. 2 Technical Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 Chemical Synthesis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35 2. 3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 . . . . . . . . . . . . . Chemical Synthesis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 Binding to anti-CCK antisera and CCK-B receptors . . . . . . . . . . . . . . . . . . . . . . . . . . 40 Binding to streptavidin agarose and photoelution . . . . . . . . . . . . . . . . . . . . . . . . . . . 40 Affinity chromatography . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41 2. 4 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44 . . . . . . . . . . . . . . . . . Purification of the Receptor for Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) using Biotinylated Ligands . . . . . . . . . . . . . . . . . . . . . . . . . 45 Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45 3. 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46 . . . . . . . . . . . . . . . . . 3. 2 Technical Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 Preparation of a Biotinylated Ligand . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 Preparation of Membrane Fraction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50 Solubilization of the PACAP Receptor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51 Partial Purification of the PACAP Receptor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52 Affinity Purification of the PACAP Receptor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54 Final Purification of the PACAP Receptor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 Receptor-Binding Assay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57 3. 3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 . . . . . . . . . . . . . 3. 4 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61 Impurity in PACAP27-Cys-NH. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61 2 Low specific activity of the PACAP receptor despite a single band . . . . . . . . . . . . . 62 Acknowledgments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63 . . . . . . . . . . . . . . . . . Photoreactive Biotinylated Peptide Ligands for Affinity Labeling . . . . . . . . . 65 Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65 4. 1 Introduction. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66 . 4. 2 Technical Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . This item ships from multiple locations. Your book may arrive from Roseburg,OR, La Vergne,TN.
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Herausgegeben von Meier, T. Fahrenholz, F. (?):

A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis (?)

ISBN: 9783034873512 (?) bzw. 3034873514, in Deutsch, Birkhäuser, Taschenbuch, neu

Fr. 78.38 ( 71.95)¹(versandkostenfrei, unverbindlich)
Versandkostenfrei
buecher.de GmbH & Co. KG, [1]
2.1 Introduction ........................................................... 32 2.2 Technical Procedures ................................................... 32 Chemical Synthesis ..................................................... 35 2.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 38 . . . . . . . . . . . . . Chemical Synthesis ..................................................... 38 Binding to anti-CCK antisera and CCK-B receptors .......................... 40 Binding to streptavidin agarose and photoelution ........................... 40 Affinity chromatography ................................................. 41 2.4 Troubleshooting....................................................... 43 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 44 . . . . . . . . . . . . . . . . . Purification of the Receptor for Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) using Biotinylated Ligands ......................... 45 Summary ............................................................. 45 3.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . 46 . . . . . . . . . . . . . . . . . 3.2 Technical Procedures ................................................... 48 Preparation of a Biotinylated Ligand ...................................... 48 Preparation of Membrane Fraction ........................................ 50 Solubilization of the PACAP Receptor ..................................... 51 Partial Purification of the PACAP Receptor ................................. 52 Affinity Purification of the PACAP Receptor ................................ 54 Final Purification of the PACAP Receptor .................................. 56 Receptor-Binding Assay ................................................. 57 3.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 58 . . . . . . . . . . . . . 3.4 Troubleshooting ....................................................... 61 Impurity in PACAP27-Cys-NH............................................ 61 2 Low specific activity of the PACAP receptor despite a single band ............. 62 Acknowledgments ..................................................... 62 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 63 . . . . . . . . . . . . . . . . . Photoreactive Biotinylated Peptide Ligands for Affinity Labeling ......... " 65 Summary ............................................................. 65 4.1 Introduction................................................ . . . . . . . .. . 66 . 4.2 Technical Procedures ................................................. " 67 Synthesis of a trifunctional photoactivatable biotinylating reagent ............. 67 Synthesis of photo reactive biotinylated peptide hormones ................... 67 Site-specific incorporation of biotin and photo labels in separate steps ........ 69 Photoaffinity labeling ................................................... 72 4.3 Results and Discussion .................................................. 76 Synthesis of photoactivatable insulins with permanent biotin labels ............ 76 Applications: Insulin .................................................... 78 VI Examples for other applications .......................................... 78 4.4 Troubleshooting....................................................... 79 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81 . . . . . . . . . . . . . . . . . . .Softcover reprint of the original 1st ed. 1996. 2012. 244 mmVersandfertig in 3-5 Tagen, Softcover
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Falk Fahrenholz (?):

A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis (2012) (?)

ISBN: 9783034873512 (?) bzw. 3034873514, in Deutsch, Birkhäuser Mrz 2012, Taschenbuch, neu, Nachdruck

Fr. 93.19 ( 85.55)¹ + Versand: Fr. 16.88 ( 15.50)¹ = Fr. 110.07 ( 101.05)¹(unverbindlich)
Von Händler/Antiquariat, AHA-BUCH GmbH [51283250], Einbeck, Germany
This item is printed on demand - Print on Demand Titel. Neuware - 2.1 Introduction . 32 2.2 Technical Procedures . 32 Chemical Synthesis . 35 2.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 . . . . . . . . . . . . . Chemical Synthesis . 38 Binding to anti-CCK antisera and CCK-B receptors . 40 Binding to streptavidin agarose and photoelution . 40 Affinity chromatography . 41 2.4 Troubleshooting. 43 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44 . . . . . . . . . . . . . . . . . Purification of the Receptor for Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) using Biotinylated Ligands . 45 Summary . 45 3.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46 . . . . . . . . . . . . . . . . . 3.2 Technical Procedures . 48 Preparation of a Biotinylated Ligand . 48 Preparation of Membrane Fraction . 50 Solubilization of the PACAP Receptor . 51 Partial Purification of the PACAP Receptor . 52 Affinity Purification of the PACAP Receptor . 54 Final Purification of the PACAP Receptor . 56 Receptor-Binding Assay . 57 3.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 . . . . . . . . . . . . . 3.4 Troubleshooting . 61 Impurity in PACAP27-Cys-NH. 61 2 Low specific activity of the PACAP receptor despite a single band . 62 Acknowledgments . 62 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63 . . . . . . . . . . . . . . . . . Photoreactive Biotinylated Peptide Ligands for Affinity Labeling . ' 65 Summary . 65 4.1 Introduction. . . . . . . . . . 66 . 4.2 Technical Procedures . ' 67 Synthesis of a trifunctional photoactivatable biotinylating reagent . 67 Synthesis of photo reactive biotinylated peptide hormones . 67 Site-specific incorporation of biotin and photo labels in separate steps . 69 Photoaffinity labeling . 72 4.3 Results and Discussion . 76 Synthesis of photoactivatable insulins with permanent biotin labels . 76 Applications: Insulin . 78 VI Examples for other applications . 78 4.4 Troubleshooting. 79 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81 . . . . . . . . . . . . . . . . . . . 240 pp. Englisch
9783034873512 - Herausgegeben von Meier, T. Fahrenholz, F.: A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis
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Herausgegeben von Meier, T. Fahrenholz, F. (?):

A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis (?)

ISBN: 9783034873512 (?) bzw. 3034873514, in Deutsch, Birkhäuser, Taschenbuch, neu

Fr. 78.38 ( 71.95)¹(versandkostenfrei, unverbindlich)
Versandkostenfrei
buecher.de GmbH & Co. KG, [1]
ceptor-Binding Assay ................................................. 57 3.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 58 . . . . . . . . . . . . . 3.4 Troubleshooting ....................................................... 61 Impurity in PACAP27-Cys-NH............................................ 61 2 Low specific activity of the PACAP receptor despite a single band ............. 62 Acknowledgments ..................................................... 62 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 63 . . . . . . . . . . . . . . . . . Photoreactive Biotinylated Peptide Ligands for Affinity Labeling ......... " 65 Summary ............................................................. 65 4.1 Introduction................................................ . . . . . . . .. . 66 . 4.2 Technical Procedures ................................................. " 67 Synthesis of a trifunctional photoactivatable biotinylating reagent ............. 67 Synthesis of photo reactive biotinylated peptide hormones ................... 67 Site-specific incorporation of biotin and photo labels in separate steps ........ 69 Photoaffinity labeling ................................................... 72 4.3 Results and Discussion .................................................. 76 Synthesis of photoactivatable insulins with permanent biotin labels ............ 76 Applications: Insulin .................................................... 78 VI Examples for other applications .......................................... 78 4.4 Troubleshooting....................................................... 79 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81 . . . . . . . . . . . . . . . . . . .2012. xiv, 240 S. 244 mmVersandfertig in 3-5 Tagen, Softcover
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A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis (Paperback) (2012) (?)

ISBN: 9783034873512 (?) bzw. 3034873514, in Deutsch, Springer Basel, Switzerland, Taschenbuch, neu, Nachdruck

Fr. 102.49 ( 94.09)¹ + Versand: Fr. 1.48 ( 1.36)¹ = Fr. 103.97 ( 95.45)¹(unverbindlich)
Von Händler/Antiquariat, The Book Depository EURO [60485773], London, United Kingdom
Language: English Brand New Book ***** Print on Demand *****.2.1 Introduction .32 2.2 Technical Procedures .32 Chemical Synthesis .35 2.3 Results and Discussion.38 .Chemical Synthesis .38 Binding to anti-CCK antisera and CCK-B receptors .40 Binding to streptavidin agarose and photoelution .40 Affinity chromatography .41 2.4 Troubleshooting.43 References .44 .Purification of the Receptor for Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) using Biotinylated Ligands .45 Summary .45 3.1 Introduction .46 .3.2 Technical Procedures .48 Preparation of a Biotinylated Ligand .48 Preparation of Membrane Fraction .50 Solubilization of the PACAP Receptor .51 Partial Purification of the PACAP Receptor .52 Affinity Purification of the PACAP Receptor .54 Final Purification of the PACAP Receptor .56 Receptor-Binding Assay .57 3.3 Results and Discussion.58 .3.4 Troubleshooting .61 Impurity in PACAP27-Cys-NH.61 2 Low specific activity of the PACAP receptor despite a single band .62 Acknowledgments .62 References .63 .Photoreactive Biotinylated Peptide Ligands for Affinity Labeling . 65 Summary .65 4.1 Introduction.66 . 4.2 Technical Procedures . 67 Synthesis of a trifunctional photoactivatable biotinylating reagent .67 Synthesis of photo reactive biotinylated peptide hormones .67 Site-specific incorporation of biotin and photo labels in separate steps .69 Photoaffinity labeling .72 4.3 Results and Discussion .76 Synthesis of photoactivatable insulins with permanent biotin labels .76 Applications: Insulin .78 VI Examples for other applications .78 4.4 Troubleshooting.79 References .81 . Softcover reprint of the original 1st ed. 1996.