Isolation and Molecular Characterization of Avian metapneumovirus: Swollen head syndrome (SHS) - A consequence of infection of chickens with avian metapneumovirus (Paperback)
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Isolation and Molecular Characterization of Avian metapneumovirus
DE NWISBN: 9783659745607 bzw. 365974560X, in Deutsch, neu.
Lieferung aus: Deutschland, Lieferzeit: 6 Tage.
A study was undertaken to isolate and characterize avian metapneumovirus (aMPV) causing swollen head syndrome in chickens. Out of 21 oropharyngeal swab samples collected, only one sample from eight weeks old commercial layers was positive for aMPV N gene specific RT-PCR. The positive sample was inoculated in vero cells and isolated after four blind passages. The virus was identified by Agar gel precipitation test with reference aMPV hyperimmune serum. Further it was confirmed by N gene specific RT-PCR. Molecular subtyping based on G gene specific RT PCR revealed that the isolate belong to aMPV subtype A. The isolate was shown to have a homology of 96.4 per cent with the European isolate (8544) and 89.7 to 100 per cent with other isolates of Europe, South Korea and Brazil (SHS/BR/669/03). Seroprevalence study of 1745 serum samples collected from 69 flocks using ELISA revealed that 925 samples from 48 flocks (69.56 %) were positive for aMPV antibodies with a GMT of 3513±114. The inactivated mineral oil adjuvant vaccine prepared from the isolate induced effective seroconversion after booster vaccination.
A study was undertaken to isolate and characterize avian metapneumovirus (aMPV) causing swollen head syndrome in chickens. Out of 21 oropharyngeal swab samples collected, only one sample from eight weeks old commercial layers was positive for aMPV N gene specific RT-PCR. The positive sample was inoculated in vero cells and isolated after four blind passages. The virus was identified by Agar gel precipitation test with reference aMPV hyperimmune serum. Further it was confirmed by N gene specific RT-PCR. Molecular subtyping based on G gene specific RT PCR revealed that the isolate belong to aMPV subtype A. The isolate was shown to have a homology of 96.4 per cent with the European isolate (8544) and 89.7 to 100 per cent with other isolates of Europe, South Korea and Brazil (SHS/BR/669/03). Seroprevalence study of 1745 serum samples collected from 69 flocks using ELISA revealed that 925 samples from 48 flocks (69.56 %) were positive for aMPV antibodies with a GMT of 3513±114. The inactivated mineral oil adjuvant vaccine prepared from the isolate induced effective seroconversion after booster vaccination.
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Isolation and Molecular Characterization of Avian metapneumovirus: Swollen head syndrome (SHS) - A consequence of infection of chickens with avian metapneumovirus (Paperback) (2015)
DE PB NW RPISBN: 9783659745607 bzw. 365974560X, in Deutsch, LAP Lambert Academic Publishing, Taschenbuch, neu, Nachdruck.
Von Händler/Antiquariat, The Book Depository EURO [60485773], London, United Kingdom.
Language: English Brand New Book ***** Print on Demand *****.
Language: English Brand New Book ***** Print on Demand *****.
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Isolation and Molecular Characterization of Avian Metapneumovirus (2015)
EN PB NWISBN: 9783659745607 bzw. 365974560X, in Englisch, 152 Seiten, LAP Lambert Academic Publishing, Taschenbuch, neu.
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Von Händler/Antiquariat, Book Depository CA.
Die Beschreibung dieses Angebotes ist von geringer Qualität oder in einer Fremdsprache. Trotzdem anzeigen
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Isolation and Molecular Characterization of Avian metapneumovirus
DE NWISBN: 9783659745607 bzw. 365974560X, in Deutsch, LAP Lambert Academic Publishing, neu.
Die Beschreibung dieses Angebotes ist von geringer Qualität oder in einer Fremdsprache. Trotzdem anzeigen
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